Dendritic cell-targeted lentiviral vector development
Lentiviral vectors have been highly useful both in research and gene therapy approaches to therapy because of their ability to stably express genes in a wide variety of target cell-types. However, the vectors don’t work well in DCs because SAMHD1 blocks them. We devised HIV-1-based lentiviral vectors that package SIV Vpx, thereby overcoming the block to infection. Our vectors transduce DCs in tissue culture with a two-log increase in titer. This high efficiency transduction provides a means by which DCs can now be used in gene therapy.